check_url/66863?article_type=b&slug=high-resolution-analysis-of-mitochondrial-function-in-human-pbmcs

High-Resolution Analysis of Mitochondrial Function in Human PBMCs

May 24, 2024
check_url/66863?article_type=b&slug=high-resolution-analysis-of-mitochondrial-function-in-human-pbmcs

Højopløselig fluorespirometri til vurdering af dynamiske ændringer i mitokondriemembranpotentiale i humane immunceller

01:24 min
•
check_url/66863?article_type=b&slug=high-resolution-analysis-of-mitochondrial-function-in-human-pbmcs

Magnetisk adskillelse af CD14+ og CD3+ celler og fluorespirometri til undersøgelse af mitokondriel dynamik

05:58 min
•
, , ,
1Division of Metabolism, Endocrinology and Nutrition, Department of Medicine,University of Washington, 2Department of Radiology,University of Washington, 3Department of Laboratory Medicine and Pathology,University of Washington

Summary

Automatic Translation

Metoder til at studere mitokondriel bioenergetik under fysiologisk relevante substratkoncentrationer i immunceller er begrænsede. Vi leverer en detaljeret protokol, der bruger højopløsningsfluorespirometri til at vurdere ændringer i mitokondriemembranpotentialets respons på energibehov i humane T-celler, monocytter og perifere mononukleære celler.

Article