Single-Cell RNA Sequencing of Mutant Whole Mouse Embryos: From the Epiblast to the End of Gastrulation

Elizabeth Abraham; Mikel Zubillaga; Thomas Roule; Eleonora Stronati; Naiara Akizu; Conchi Estaras

doi:10.3791/66866

Isolation and Genotyping of Gastrulating Mouse Embryo for Single-Cell Sequencing

June 14, 2024

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

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Elizabeth Abraham, Mikel Zubillaga, Thomas Roule, Eleonora Stronati, Naiara Akizu, Conchi Estaras

¹Department of Cardiovascular Sciences, Aging + Cardiovascular Discovery Center,Temple University, Lewis Katz School of Medicine, ²Raymond G. Perelman Center for Cellular and Molecular Therapeutics,The Children’s Hospital of Philadelphia, ³Department of Child and Adolescence Psychiatry,Children’s Hospital of Philadelphia

Summary

This paper establishes a pipeline for high-quality single-cell and nuclei suspensions of gastrulating mouse embryos for sequencing of single cells and nuclei.

Isolation and Genotyping of Gastrulating Mouse Embryo for Single-Cell Sequencing

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Summary

Article