Generation and Purification of Chromosome Conformation Capture (3C) Library (Video) | JoVE

Published: May 12, 2023

doi:

10.3791/200093

Acadia Joniec*¹, Joseph Leszczynski*¹, Sokhna Ndoye*¹, Jillian Sylvia*¹, Steven E. Weicksel²

¹Department of Biological and Biomedical Sciences,Bryant University, ²Center of Health and Behavioral Sciences,Bryant University

Tags

Keywords: Chromosome Conformation Capture 3C Library Caenorhabditis Elegans Chromatin Cross-linking Nuclei Collection DpnII Restriction Enzyme Chromatin Digestion Chromatin Ligation T4 DNA Ligase Protein Digestion Reverse Cross-linking Proteinase K RNase A Phenol-chloroform Extraction Purification

Play Video

PDF

DOI

Cite This Article

Generation and Purification of Chromosome Conformation Capture (3C) Library. J. Vis. Exp. (Pending Publication), e200093, doi:10.3791/200093 (2023).

Generation and Purification of Chromosome Conformation Capture (3C) Library (Video) | JoVE

Tags

Play Video

Cite This Article

View Video

✖

To prove you're not a robot, please enter the text in the image below