Transfection and Transduction of Murine T-Cells for CAR-T Cell Generation - JoVE

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Transfection and Transduction of Murine T-Cells for CAR-T Cell Generation (Video) | JoVE

Published: February 02, 2024

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Rosa L. Vincent, Fangda Li, Edward R. Ballister, Nicholas Arpaia³, Tal Danino^3,4

¹Department of Biomedical Engineering,Columbia University, ²Department of Microbiology & Immunology, Vagelos College of Physicians and Surgeons,Columbia University, ³Herbert Irving Comprehensive Cancer Center,Columbia University, ⁴Data Science Institute,Columbia University

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Keywords: Murine T-cells CAR-T Cell Generation Transfection Transduction Phoenix Eco Plate Viral Supernatant Splenocytes CD3 Positive T-cells Negative Selection T-cell Activation Human Fibronectin Transduction Enhancer

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Transfection and Transduction of Murine T-Cells for CAR-T Cell Generation. J. Vis. Exp. (Pending Publication), e200881, doi:10.3791/200881 (2024).

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