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Preparation and Testing of Multiplex R3T One-Step RT-qPCR Kit Components (Video) | JoVE

Published: November 10, 2023

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Atheer Althobaiti, Kareem Hamdan, Mohamed A. Sobhy, Renad Rawas, Masateru Takahashi, Olga Artyukh, Muhammad Tehseen

¹Biological and Environmental Sciences and Engineering Division (BESE),King Abdullah University of Science and Technology (KAUST)

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RT-qPCR Multiplex SARS-CoV-2 Influenza A Influenza B MERS-CoV Primer Probe Enzyme Mix Taq Polymerase M-MLV RT Viral RNA Synthetic RNA RNA Copies 96-well Plate Real-time PCR Amplification Efficiency

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Preparation and Testing of Multiplex R3T One-Step RT-qPCR Kit Components. J. Vis. Exp. (Pending Publication), e201009, doi:10.3791/201009 (2023).

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