JoVE Journal > Bioengineering

FPLC Purification and Characterization of Recombinant AAV

Published: April 05, 2024

doi:

Miguel M. Lopes*^1,2,3, Sara M. Lopes*^1,2,3, Rafael Baganha^2,4, Carina Henriques^2,5, Ana C. Silva^2,3, Diana D. Lobo^2,3, Luísa Cortes^2,3,6, Luís Pereira de Almeida*^1,2,4,5, Rui Jorge Nobre*^1,2,3,4

¹CNC-UC – Center for Neuroscience and Cell Biology,University of Coimbra, ²CIBB – Center for Innovative Biomedicine and Biotechnology,University of Coimbra, ³IIIUC – Institute for Interdisciplinary Research,University of Coimbra, ⁴ViraVector – Viral Vectors for Gene Transfer Core Facility,University of Coimbra, ⁵FFUC – Faculty of Pharmacy,University of Coimbra, ⁶MICC-CNC – Microscopy Imaging Center of Coimbra – CNC,University of Coimbra

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Keywords: FPLC Purification Recombinant AAV Heparin Column Buffer A Buffer B Sample Application Column Equilibration Sample Elution Fraction Collection Buffer Exchange Concentration

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FPLC Purification and Characterization of Recombinant AAV. J. Vis. Exp. (Pending Publication), e201699, doi:10.3791/201699 (2024).

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