Multiomics Analysis of <em>TMEM200A</em> as a Pan-Cancer Biomarker

Yaowen Zhang; Shanshan Kuang; Hancheng Qin; Na Zhao; Yixin Yang; Jisheng Xie

doi:10.3791/65795

TMEM200A: A Novel Oncogene Regulating EMT and PI3K/AKT in Gastric Cancer

September 15, 2023

Multiomics Analysis of TMEM200A as a Pan-Cancer Biomarker

01:14 min

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Transfection of TMEM200A siRNA in Stomach Adenocarcinoma Cell Lines

01:21 min

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Quantification of mRNA in TMEM200A siRNA Transfected Stomach Adenocarcinoma Cells

01:33 min

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Protein Detection in TMEM200A siRNA Transfected Stomach Adenocarcinoma Cells Using Western Blotting

02:49 min

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Determining the Cell Viability of TMEM200A siRNA Transfected HGC-27 Cell Lines Using CCK-8 Assays

00:57 min

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Yaowen Zhang, Shanshan Kuang, Hancheng Qin, Na Zhao, Yixin Yang, Jisheng Xie

¹Department of Histology and Embryology,Youjiang Medical University for Nationalities, ²Department of Pathophysiology,Youjiang Medical University for Nationalities

Summary

Here, a protocol is presented in which multiple bioinformatic tools are combined to study the biological functions of TMEM200A in cancer. In addition, we also experimentally validate the bioinformatics predictions.