Application of Monolayer Graphene to Cryo-Electron Microscopy Grids for High-resolution Structure Determination

Andrew V. Grassetti; Mira B. May; Joseph  H. Davis

doi:10.3791/66023

Coating CryoEM Grids with Monolayer Graphene to Improve Cryo-Sample Preparation

November 10, 2023

Author Spotlight: Enhancing CryoEM Sample Preparation Using Graphene Monolayer on Microscopy Grids

01:38 min

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Preparing MMA-Coated CVD Graphene for Fabrication of Graphene-Coated CryoEM Grids

01:54 min

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Preparing Grid-Sized MMA-Coated CVD Graphene Squares for Attaching to CryoEM Grids

01:49 min

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Fabricating Graphene-Coated Cryo-EM Grids Using Grid-Sized MMA-Coated CVD Graphene Squares

02:43 min

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Andrew V. Grassetti*¹, Mira B. May*¹, Joseph H. Davis²

¹Department of Biology,Massachusetts Institute of Technology, ²Program in Computational and Systems Biology,Massachusetts Institute of Technology

Summary

The application of support layers to cryogenic electron microscopy (cryoEM) grids can increase particle density, limit interactions with the air-water interface, reduce beam-induced motion, and improve the distribution of particle orientations. This paper describes a robust protocol for coating cryoEM grids with a monolayer of graphene for improved cryo-sample preparation.