JOVE JOURNAL

生物化学

Showing 217 - 228 in 731 videos.

In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays

Ruchir C. Bobde*^1,2, Ketul Saharan*^1,2, Somanath Baral³, Surajit Gandhi², Archana Samal², Rajivgandhi Sundaram, Ashish Kumar⁴, Ajit K. Singh⁵, Aritreyee Datta, Dileep Vasudevan

¹Institute of Life Sciences, ²Regional Centre for Biotechnology, ³School of Biotechnology,KIIT University, ⁴Department of Molecular Biophysics and Biochemistry,Yale University, ⁵Department of Pharmacology,University of Vermont College of Medicine

Journal (Biochemistry)

Purification of Endogenous Drosophila Transient Receptor Potential Channels

Jia Liu*¹, Yuyang Liu*¹, Weidi Chen, Yuzhen Ding, Xiaoru Lan, Wei Liu

¹Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute,Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center

Journal (Biochemistry)

Modeling an Enzyme Active Site using Molecular Visualization Freeware

Kristen Procko, Sandy Bakheet, Josh T. Beckham, Margaret A. Franzen, Henry Jakubowski, Walter R. P. Novak

¹The University of Texas at Austin, ²Mount Mary University, ³College of St. Benedict/St. John’s University, ⁴Wabash College

Journal (Biochemistry)

Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans

Jelle Molenkamp*¹, Anna den Outer*¹, Vera van Schijndel*¹, Tessa Sinnige

¹Bijvoet Centre for Biomolecular Research,Utrecht University

Journal (Biochemistry)

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Katherina Hemmen*¹, Susobhan Choudhury*¹, Mike Friedrich, Johannes Balkenhol, Felix Knote, Martin J. Lohse, Katrin G. Heinze

¹Rudolf Virchow Center for Integrative and Translation Bioimaging,Julius-Maximilian University Wuerzburg, ²Max Delbrück Center for Molecular Medicine, Berlin

Journal (Biochemistry)

Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System

James B. Pace, Nian N. Huang, Jacqueline P. Séguin, Candi Esquina, Erin Olin, Guangyu Zhu, Grant Carr

¹Discovery Biology,Curia Global Inc.

Journal (Biochemistry)

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Sergey I. Kovalchuk, Rustam Ziganshin, Irina Shelukhina

¹Laboratory of Bioinformatics Methods in Combinatorial Chemistry and Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences, ²Department of Molecular Neuroimmune Signalling, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences

Journal (Biochemistry)

Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography

Jana Moravcová, Matyáš Pinkas, Radka Holbová, Jiří Nováček

¹Central European Institute of Technology,Masaryk University

Journal (Biochemistry)

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

Chloe D. Truong², Dewight R. Williams, Mary Zhu, Joseph Che-Yen Wang, Po-Lin Chiu²

¹School of Molecular Sciences,Arizona State University, ²Center for Applied Structural Discovery, Biodesign Institute,Arizona State University, ³Eyring Materials Center,Arizona State University, ⁴Department of Microbiology and Immunology,The Pennsylvania State University

Journal (Biochemistry)

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Yue Zhang*¹, Peng Cao*², Fang Lu, Jinjun Cheng, Huihua Qu

¹School of Life Science,Beijing University of Chinese Medicine, ²Third Affiliated Hospital, Beijing University of Chinese Medicine, ³National Institute of TCM Constitution and Preventive Medicine,Beijing University of Chinese Medicine, ⁴Center of Scientific Experiment,Beijing University of Chinese Medicine

Journal (Biochemistry)

Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy

David P. Klebl, Frank Sobott, Howard D. White, Stephen P. Muench

¹School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology,University of Leeds, ²School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology,University of Leeds, ³Department of Physiological Sciences,Eastern Virginia Medical School

Journal (Biochemistry)

In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase

Victoria Lucia Alonso², Romina Manarin, Virginia Perdomo, Ernesto Gulin, Esteban Serra², Pamela Cribb²

¹Área Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas,Universidad Nacional de Rosario (UNR), ²Instituto de Biología Molecular y Celular de Rosario (IBR),CONICET, ³Instituto de Investigaciones Biomédicas (INBIOMED),Universidad de Buenos Aires (UBA) Facultad de Medicina, CONICET

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