Shandong University of Traditional Chinese Medicine 4 articles published in JoVE Immunology and Infection Du-Moxibustion in a Mouse Model of Ankylosing Spondylitis Wen-Yan Yu1, Xu-Hao Li1, Zhi-Xia Jia2, Yi Hou1, Tian-tian Dong3, Xing-Xin Wang1, Yuan-Xiang Liu2, Ji-Guo Yang1 1School of Acupuncture-Moxibustion and Tuina, Shandong University of Traditional Chinese Medicine, 2School of First Clinical Medicine, Shandong University of Traditional Chinese Medicine, 3External Treatment Center, Affiliated Hospital of Shandong University of Traditional Chinese Medicine This paper describes in detail the operation procedures and precautions to be taken during Du-moxibustion in the treatment of ankylosing spondylitis in experimental mice. Medicine Tuina Intervention in Rabbit Model of Knee Osteoarthritis Shidong Zhang*1, Xuan Zhang*2, Gangyi Sun3, Kai Wang1, Yingjie Qiao1, Yanling He3, Muzhen Li1, Huadong Li3, Lijun Zheng3 1Shandong University of Traditional Chinese Medicine, 2Liaoning University of Traditional Chinese Medicine, 3Shandong University of Traditional Chinese Medicine Affiliated Hospital The protocol describes a method for Tuina intervention in a rabbit model of knee osteoarthritis. Medicine Tuina in a Frozen Shoulder Rat Model: An Efficient and Reproducible Protocol Yingjie Qiao1, Yanhong Yang2, Jianmin Wang3, Muzhen Li1, Lijun Zheng1, Huadong Li3, Shidong Zhang1 1College of Acupuncture and Tuina, Shandong University of Traditional Chinese Medicine, 2Department of Tuina, Jinan Massage Hospital, 3Department of Tuina, Shandong University of Traditional Chinese Medicine Affiliated Hospital This study develops an efficient and reproducible Tuina protocol for treating frozen shoulder established in a rat model. This approach will help to study the Tuina therapy treatment method for frozen shoulders. Biochemistry An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause Bing Sun1, Yue-zhang Yin2, Jing Xiao1 1Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences & Peking Union Medical College, 2Shandong University of Traditional Chinese Medicine Clinically, estrogen deficiency in menopausal women may aggravate the incidence of lipid disruption and atherosclerosis. We established an in vivo estrogen deficiency model by bilateral ovariectomy via a double dorsal-lateral incision in apoE-/- mice. The mouse model is applicable for screening exogenous estrogen treatments of cardiovascular dysfunction after menopause.