Liv Hospital 2 articles published in JoVE Bioengineering Preparation and Characterization of Graphene-Based 3D Biohybrid Hydrogel Bioink for Peripheral Neuroengineering Asli Pinar Zorba Yildiz1,2,4, Hakan Darici3,4,5, Burcak Yavuz2,4,6, Emrah Sefik Abamor2, Ceren Ozdemir2,5, Muge Elif Yasin4, Melahat Bagirova2,7, Adil Allahverdiyev2,7, Erdal Karaoz3,4,5,8 1Vocational School of Health Services, Istinye University, 2Chemical Metallurgy Faculty, Yildiz Technical University, 3Faculty of Medicine, Department of Histology & Embryology, Istinye University, 43D Bioprinting Design & Prototyping R&D Center, Istinye University, 5Stem Cell, and Tissue Engineering R&D Center, Istinye University, 6Vocational School of Health Services, Altinbas University, 7The V. Akhundov National Scientific Research Medical Prophylactic Institute, 8Center for Stem Cells and Regenerative Medicine (LivMedCell), Liv Hospital In this manuscript, we demonstrate the preparation of a biohybrid hydrogel bioink containing graphene for use in peripheral tissue engineering. Using this 3D biohybrid material, the neural differentiation protocol of stem cells is performed. This can be an important step in bringing similar biomaterials to the clinic. Bioengineering Formulating and Characterizing an Exosome-based Dopamine Carrier System Burçak Yavuz1,2, Hakan Darici3,4,5,6, Aslı Pınar Zorba Yildiz2,7, Emrah Şefik Abamor2, Murat Topuzoğullari2, Melahat Bağirova8, Adil Allahverdiyev8, Erdal Karaoz3,4,5,6,9 1Vocational School of Health Services/İstanbul, Altinbas University, 2Chemical Metallurgy Faculty, Department of Bioengineering, Yildiz Technical University, 3Faculty of Medicine, Department of Histology & Embryology, Istinye University, 43D Bioprinting Design & Prototyping R&D Center, Istinye University, 5Stem Cell and Tissue Engineering R&D Center, Istinye University, 6Medicine Faculty, Istinye University, 7Vocational School of Health Services, Istinye University, 8The V. Akhundov National Scientific Research Medical Prophylactic Institute, 9Center for Stem Cells and Regenerative Medicine (LivMedCell), Liv Hospital Here, we aimed to obtain a formulation by dopamine loading of the isolated exosomes of stem cells from Wharton's jelly mesenchymal stem cells. Exosome isolation and characterization, drug loading into the resulting exosomes, and the cytotoxic activity of the developed formulation are described in this protocol.