Universitat Innsbruck 1 article published in JoVE Environment Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples Rudolf Markt1, Mathias Wunderer1, Eva Maria Prem1, Mira Mutschlechner1, Nina Lackner2, Andreas Otto Wagner1 1Department of Microbiology, Universität Innsbruck, 2Department of Hygiene and Medical Microbiology, Medical University of Innsbruck A method for the extraction of cofactor F420 from pure cultures was optimized for the liquid chromatographic separation and analysis of F420 tail length in pure culture and environmental samples.