University of Goettingen 5 articles published in JoVE Behavior The Collective Trust Game: An Online Group Adaptation of the Trust Game Based on the HoneyComb Paradigm Marie Ritter1, Charlotte F. Kroll*1, Henriette Voigt*1, Johannes Pritz1, Margarete Boos1 1University of Goettingen The Collective Trust Game is a computer-based, multi-agent trust game based on the HoneyComb paradigm, which enables researchers to assess the emergence of collective trust and related constructs, such as fairness, reciprocity, or forward-signaling. The game allows detailed observations of group processes through movement behavior in the game. Bioengineering Fibroblast Derived Human Engineered Connective Tissue for Screening Applications Gabriela L. Santos1,2, Tim Meyer1,2, Malte Tiburcy1,2, Alisa DeGrave1,2, Wolfram-Hubertus Zimmermann1,2,3,4,5, Susanne Lutz1,2 1Institute of Pharmacology and Toxicology, University Medical Center Goettingen, 2DZHK (German Center for Cardiovascular Research) partner site, Goettingen, 3Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC), University of Goettingen, 4Center for Neurodegenerative Diseases (DZNE), 5Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP) Presented here is a protocol to generate engineered connective tissues for a parallel culture of 48 tissues in a multi-well plate with double poles, suitable for mechanistic studies, disease modeling, and screening applications. The protocol is compatible with fibroblasts from different organs and species and is exemplified here with human primary cardiac fibroblasts. Bioengineering Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Fitzwilliam Seibertz1,2, Martyn Reynolds3, Niels Voigt1,2,4 1Institute of Pharmacology and Toxicology, University Medical Center Goettingen, Goettingen, Germany, 2DZHK (German Center for Cardiovascular Research), Partner Site Goettingen, Germany, 3Cairn Research Ltd, Faversham, United Kingdom, 4Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Goettingen, Germany Here we describe optical acquisition and characterization of action potentials from induced pluripotent stem cell derived cardiomyocytes using a high-speed modular photometry system. Behavior The HoneyComb Paradigm for Research on Collective Human Behavior Margarete Boos1, Johannes Pritz2, Michael Belz3 1Institute for Psychology, University of Goettingen, 2Courant Research Centre Evolution of Social Behavior, University of Goettingen, 3Department of Psychiatry and Psychotherapy, University Medical Centre Goettingen Here, we present the computer-based, multi-agent game HoneyComb, which enables experimental investigations of collective human movement behavior via black-dot-avatars on a virtual 2D hexagonal playfield. Different experimental conditions, like variable incentives on goal fields or vision radius, can be set, and their effects on human movement behavior can be investigated. Neuroscience Optogenetic Stimulation of the Auditory Nerve Victor H. Hernandez1,2,5, Anna Gehrt1,3, Zhizi Jing3, Gerhard Hoch1, Marcus Jeschke1, Nicola Strenzke3, Tobias Moser1,2,4 1InnerEarLab, Department of Otolaryngology, University Medical Center Goettingen, 2Bernstein Focus for Neurotechnology, University of Goettingen, 3Auditory Systems Physiology Group, Department of Otolaryngology, University Medical Center Goettingen, 4Center for Nanoscale Microscopy and Molecular Physiology of the Brain, University of Goettingen, 5Department of Chemical, Electronic, and Biomedical Engineering, University of Guanajuato Cochlear implants (CIs) enable hearing by direct electrical stimulation of the auditory nerve. However, poor frequency and intensity resolution limits the quality of hearing with CIs. Here we describe optogenetic stimulation of the auditory nerve in mice as an alternative strategy for auditory research and developing future CIs.