Complutense University of Madrid 7 articles published in JoVE Biology Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ Jesús Espada1,2, Elisa Carrasco3, María I. Calvo-Sánchez1,4, Sandra Fernández-Martos1, Juan José Montoya5 1Ramón y Cajal Institute for Health Research (IRYCIS), Ramón y Cajal University Hospital, 2 The aim of this protocol is to induce transient in vivo production of nonlethal levels of reactive oxygen species (ROS) in mouse skin, further promoting physiological responses in the tissue. Behavior Stereoacuity Improvement using Random-Dot Video Games Santiago Martín-González1, Juan Portela-Camino2, Javier Ruiz-Alcocer3, Igor Illarramendi-Mendicute4, Rafaela Garrido-Mercado5 1Department of Construction and Manufacturing Engineering, University of Oviedo, 2Department of Optometry, Clinic Begira, 3Department of Health Sciences, Complutense University of Madrid, 4Department of Optometry, Begitek Clinic, 5School of Optometry, Complutense University of Madrid Presented here is a protocol to improve stereoacuity using gamified perceptual learning software based on random-dot stimuli. Patients are stereo-deficient subjects without strabismus. The protocol combines optometry center visits with home exercises using software. Compliance and stereoacuity evolution are stored in the cloud. Medicine Combining Augmented Reality and 3D Printing to Display Patient Models on a Smartphone Rafael Moreta-Martinez1,2, David García-Mato1,2, Mónica García-Sevilla1,2, Rubén Pérez-Mañanes2,3,4, José A. Calvo-Haro2,3,4, Javier Pascau1,2 1Department of Bioengineering and Aerospace Engineering, Universidad Carlos III de Madrid, 2Instituto de Investigación Sanitaria Gregorio Marañón, 3Department of Orthopedic Surgery and Traumatology, Hospital General Universitario Gregorio Marañón, 4Department of Surgery, Faculty of Medicine, Universidad Complutense de Madrid Presented here is a method to design an augmented reality smartphone application for the visualization of anatomical three-dimensional models of patients using a 3D-printed reference marker. Neuroscience Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations Rosa Gómez-Villafuertes*1,2,3, Lucía Paniagua-Herranz*1,2,3, Sergio Gascon*4,5, David de Agustín-Durán1,2,3, María de la O Ferreras1,2,3, Juan Carlos Gil-Redondo1,2,3, María José Queipo1,2,3, Aida Menendez-Mendez1,2,3, Ráquel Pérez-Sen1,2,3, Esmerilda G. Delicado1,2,3, Javier Gualix1,2,3, Marcos R. Costa6, Timm Schroeder7, María Teresa Miras-Portugal1,2,3, Felipe Ortega1,2,3 1Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University, 2University Institute for Neurochemistry Research (IUIN), 3Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), 4Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg/Munich, Germany Physiological Genomics, Biomedical Center, Ludwig-Maximilians University Munich, 5Toxicology and Pharmacology Department, Faculty of Veterinary medicine, Complutense University, 6Brain Institute, Federal University of Rio Grande do Norte, 7Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich A robust protocol to monitor neural populations by time-lapse video-microscopy followed by software-based post-processing is described. This method represents a powerful tool to identify biological events in a selected population during live imaging experiments. Biology A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice Chrstine Bäuerl*1, Özgun C.O. Umu*2, Pablo E. Hernandez3, Dzung B. Diep4, Gaspar Pérez-Martínez1 1Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), 2Department of Food Safety and Infection Biology, Norwegian University of Life Sciences (NMBU), 3Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid (UCM), 4Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU) Bacteriocins are believed to play a key role in defining microbial diversity in different ecological niches. Here, we describe an efficient procedure to assess how bacteriocins affect gut microbiota composition in an animal model. Biochemistry Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function Francisco J. Fernández1,2,3, Sara Gómez1, Sergio Navas-Yuste1, Miguel López-Estepa1, M. Cristina Vega1 1Department of Physical Chemical Biology, Center for Biological Research (CIB-CSIC), Spanish National Research Council (CSIC), 2Department of Immunology, Complutense University School of Medicine, 3Abvance Biotech srl A protocol is presented for the production of tRNA(UUU) and the analysis of tRNA(UUU) in complex with the enzyme TcdA by agarose gel retardation assays. Chemistry Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles Shuai Nie1, Elena Benito-Peña1,2, Huaibin Zhang1, Yue Wu3, David R. Walt1 1Department of Chemistry, Tufts University, 2Department of Analytical Chemistry, Complutense University (Spain), 3Department of Electrical and Computer Engineering, Tufts University We describe a procedure for profiling salivary proteins using multiplexed microsphere-based antibody arrays. Monoclonal antibodies were covalently linked to fluorescent dye-encoded 4.5 μm polymer microspheres using carbodiimide chemistry. The modified microspheres were deposited in fiber-optic microwells to measure protein levels in saliva using fluorescence sandwich immunoassays.