CONICET 2 articles published in JoVE Biochemistry In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase Victoria Lucia Alonso1,2, Romina Manarin1, Virginia Perdomo1, Ernesto Gulin3, Esteban Serra1,2, Pamela Cribb1,2 1Área Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), 2Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, 3Instituto de Investigaciones Biomédicas (INBIOMED), Universidad de Buenos Aires (UBA) Facultad de Medicina, CONICET We describe a high-throughput colorimetric assay measuring β-galactosidase activity in three life cycle stages of Trypanosoma cruzi, the causative agent of Chagas disease. This assay can be used to identify trypanocidal compounds in an easy, fast, and reproducible manner. Medicine Network Analysis of Foramen Ovale Electrode Recordings in Drug-resistant Temporal Lobe Epilepsy Patients Ancor Sanz-García1, Lorena Vega-Zelaya2, Jesús Pastor2, Cristina V. Torres1, Rafael G. Sola1, Guillermo J. Ortega1,3 1Neurosurgery & National Reference Unit for the Treatment of Refractory Epilepsy, Instituto de Investigación Sanitaria Hospital de la Princesa, 2Clinical Neurophysiology & National Reference Unit for the Treatment of Refractory Epilepsy, Instituto de Investigación Sanitaria Hospital de la Princesa, 3CONICET This protocol describes a procedure to track the evolution of mesial network measures in temporal lobe epilepsy (TLE) patients. It is based on the combination of intracranial recordings with a novel numerical technique for data analysis. Specifically, we present a protocol for network analyses of foramen ovale recordings.