JOVE JOURNAL

生物化学

Showing 133 - 144 in 731 videos.

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Xinghui Song, Hao Shen, Yanwei Li, Yueting Xing, Jiajia Wang, Chun Guo, Yingying Huang, Jin Chen

¹Central Laboratory of Women’s Hospital,Zhejiang University School of Medicine, ²School of Medical Technology and Information Engineering,Zhejiang Chinese Medical University, ³Core Facilities,Zhejiang University School of Medicine

Journal (Biochemistry)

Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors

Sayan Dutta Gupta, Dae-Geun Song, SueJung Lee, Jae Wook Lee, Jin-Soo Park, Chrisostomos Prodromou, Cheol-Ho Pan

¹Gangneung Institute of Natural Products,Korea Institute of Science and Technology (KIST), ²School of Life Sciences,University of Sussex

Journal (Biochemistry)

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

Isadora da Silva Zanzarini, Gustavo Barbosa, Larissa de Oliveira Prado, Ingrid Fatima Zattoni, Gustavo Da Paz, Ademir Luiz do Prado³, Waldemar Volanski, Marcos Dinís Lavarda, Fabiane Gomes de Moraes Rego, Geraldo Picheth, Vivian Rotuno Moure, Glaucio Valdameri

¹Graduate Program in Pharmaceutical Sciences, Laboratory of Cancer Drug Resistance,Federal University of Parana, ²Graduate Program in Pharmaceutical Sciences,Federal University of Parana, ³Federal Institute of Parana

Journal (Biochemistry)

Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography

Ewa Rejnowicz, Jack Wright, Margaret Veldman-Jones, Steven Harborne

¹Peak Proteins Ltd.

Journal (Biochemistry)

Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases

Eliana M. Wolpaw*¹, Marissa L. Frenett*¹, Claudia A. Mak, Sloane M. Zwanger, Madushi Raththagala

¹Department of Chemistry,Skidmore College

Journal (Biochemistry)

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

Artem Bonchuk², Nikolay Zolotarev, Konstantin Balagurov², Olga Arkova, Pavel Georgiev

¹Department of the Control of Genetic Processes, Institute of Gene Biology,Russian Academy of Sciences, ²Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology,Russian Academy of Sciences

Journal (Biochemistry)

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Sarah-Luisa Dülk, Silvia Rouskin

¹Department of Microbiology,Harvard Medical School

Journal (Biochemistry)

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

Sunday Solomon Josiah, Nur Farah Meor Azlan, Asami Oguro-Ando, Jinwei Zhang

¹Institute of Biomedical and Clinical Sciences, Medical School, Faculty of Health and Life Sciences,University of Exeter, Hatherly Laboratories

Journal (Biochemistry)

A “Dual-Addition” Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors

Caixing Xiong, Dwight Baker, Patricia Pietrantonio

¹Department of Entomology,Texas A&M University, ²Department of Biochemistry and Biophysics,Texas A&M University

Journal (Biochemistry)

A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro

Xinwen Miao*¹, Jiawei Wu*^1,2, Wenting Zhao³

¹School of Chemistry, Chemical Engineering, and Biotechnology,Nanyang Technological University, ²State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering,Zhejiang University of Technology, ³Institute for Digital Molecular Analytics and Science,Nanyang Technological University

Journal (Biochemistry)

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Jonathan T. Specker, Alexander B. Smith, Orlaith Keenan, Joseph P. Zackular³, Boone M. Prentice

¹Department of Chemistry,University of Florida, ²Division of Protective Immunity,Children’s Hospital of Philadelphia, ³Department of Pathology and Laboratory Medicine, Perelman School of Medicine,University of Pennsylvania

Journal (Biochemistry)

In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids

Julia Maldonado-Valderrama, Teresa del Castillo Santaella, Juan Antonio Holgado-Terriza, Miguel Ángel Cabrerizo-Vílchez

¹Department of Applied Physics, Faculty of Sciences, Campus de Fuentenueva,University of Granada, ²Department of Physical Chemistry, Faculty of Pharmacy, Campus de Cartuja,University of Granada, ³Department of Software Engineering, School of Computer and Telecommunication Engineering, Campus de Aynadamar,University of Granada

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