JOVE JOURNAL

生物化学

Showing 145 - 156 in 724 videos.

Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination

Yesenia Rodriguez, Kevin John Butay*¹, Kedar Sharma*¹, Elizabeth Viverette*¹, Samuel H. Wilson

¹Genome Integrity and Structural Biology Laboratory,National Institute of Environmental Health Sciences, National Institutes of Health

Journal (Biochemistry)

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Madeline M. Mumbleau*¹, Madeline R. Meyer*¹, Ming C. Hammond

¹Department of Chemistry and Henry Eyring Center for Cell & Genome Science,University of Utah

Journal (Biochemistry)

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Rebecca Switzer, Katie A. Ward, Jessica Medrano

¹Department of Chemistry,Bucknell University, ²Program in Cell Biology/Biochemistry,Bucknell University

Journal (Biochemistry)

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Brandy N. Curtis*¹, Ellysa J. D. Vogt*², Kevin S. Cannon³, Amy S. Gladfelter^2,3

¹Department of Biochemistry and Biophysics,University of North Carolina at Chapel Hill, ²Curriculum in Genetics and Molecular Biology,University of North Carolina at Chapel Hill, ³Department of Biology,University of North Carolina at Chapel Hill

Journal (Biochemistry)

Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors

Pushpanjali Soppina², Dipeshwari J. Shewale, Pradeep K. Naik, Virupakshi Soppina

¹Discipline of Biological Engineering,Indian Institute of Technology Gandhinagar, ²Department of Biotechnology and Bioinformatics,Sambalpur University

Journal (Biochemistry)

DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density

Chelcie F. Heaney², Colin J. McArdle, Kimberly F. Raab-Graham²

¹Wake Forest Translational Alcohol Research Center,Wake Forest School of Medicine, ²Physiology and Pharmacology,Wake Forest School of Medicine

Journal (Biochemistry)

Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy

Jessica L. Henty-Ridilla

¹Department of Biochemistry & Molecular Biology, and Department of Neuroscience & Physiology,State University of New York (SUNY) Upstate Medical University

Journal (Biochemistry)

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

Liza-Anastasia DiCecco*^1,2, Samantha Berry*¹, G. M. Jonaid*^1,3, Maria J. Solares⁴, Liam Kaylor⁴, Jennifer L. Gray, Carol Bator, William J. Dearnaley, Michael Spilman, Madeline J. Dressel-Dukes, Kathryn Grandfield, Sarah M. McDonald Esstman, Deborah F. Kelly^5,6

¹Department of Biomedical Engineering,Pennsylvania State University, ²Department of Materials Science and Engineering,McMaster University, ³Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences,Pennsylvania State University, ⁴Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences,Pennsylvania State University, ⁵Materials Research Institute,Pennsylvania State University, ⁶Huck Institutes of the Life Sciences,Pennsylvania State University, ⁷Applications team,Direct Electron, ⁸Application Scientist,Protochips, Inc., ⁹Department of Biology,Wake Forest University

Journal (Biochemistry)

Inner Mitochondrial Membrane Sensitivity to Na⁺ Reveals Partially Segmented Functional CoQ Pools

Pablo Hernansanz-Agustín, José Antonio Enríquez²

¹Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III CNIC, ²Centro de Investigaciones Biomédica en Red de Fragilidad y Envejecimiento, Saludable-CIBERFES

Journal (Biochemistry)

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

Satyaghosh Maurya, Vishwesh Haricharan Rai, Aditya Upasani, Saurabh Umrao², Diksha Parwana, Rahul Roy³

¹Department of Chemical Engineering,Indian Institute of Science, ²Holonyak Micro & Nanotechnology Lab,University of Illinois, Urbana-Champaign, ³Center for BioSystems Science and Engineering,Indian Institute of Science

Journal (Biochemistry)

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon

Sriram Aiyer, Timothy S. Strutzenberg, Marianne E. Bowman, Joseph P. Noel³, Dmitry Lyumkis^4,5

¹Laboratory of Genetics,The Salk Institute for Biological Studies, ²Jack H. Skirball Center for Chemical Biology and Proteomics,The Salk Institute for Biological Studies, ³Department of Chemistry and Biochemistry,University of California San Diego, ⁴Graduate School of Biological Sciences, Section of Molecular Biology,University of California San Diego, ⁵Department of Integrative Structural and Computational Biology,The Scripps Research Institute

Journal (Biochemistry)

The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples

Matthew C. Johnson*^1,2, Arshay J. Grant*¹, Ingeborg Schmidt-Krey³

¹School of Biological Sciences,Georgia Institute of Technology, ²Department of Structural Biology,Genentech, ³School of Chemistry and Biochemistry,Georgia Institute of Technology

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