JOVE JOURNAL

生物化学

Showing 457 - 468 in 731 videos.

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Yu-Ting Huang², Dinja van der Hoorn*^1,2, Leire M. Ledahawsky*^1,2, Anna A. L. Motyl*^1,2, Crispin Y. Jordan, Thomas H. Gillingwater², Ewout J. N. Groen²

¹Centre for Discovery Brain Sciences,University of Edinburgh, ²Euan MacDonald Centre for Motor Neurone Disease Research,University of Edinburgh

Journal (Biochemistry)

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization

Hadar Amartely, Daniel Some, Ayala Tsadok, Mario Lebendiker

¹Wolfson Centre for Applied Structural Biology, The Alexander Silberman Institute of Life Science,The Hebrew University of Jerusalem, ²Wyatt Technology Corporation, ³Danyel Biotech Ltd

Journal (Biochemistry)

DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes

Casey S. O’Neil, Jacie L. Beach, Todd D. Gruber

¹Department of Molecular Biology and Chemistry,Christopher Newport University

Journal (Biochemistry)

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules

Stephanie Hutin, Bart Van Laer, Christoph Mueller-Dieckmann, Gordon Leonard, Didier Nurizzo, Matthew W. Bowler

¹Structural Biology Group,European Synchrotron Radiation Facility, ²Grenoble Outstation,European Molecular Biology Laboratory

Journal (Biochemistry)

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

Stephanie Hutin*¹, Gianluca Santoni*¹, Ulrich Zander, Nicolas Foos, Sylvain Aumonier, Guillaume Gotthard, Antoine Royant³, Christoph Mueller-Dieckmann, Gordon Leonard

¹European Synchrotron Radiation Facility,Structural Biology Group, ²European Molecular Biology Laboratory, ³Univ. Grenoble Alpes, CNRS, CEA, IBS (Institut de Biologie Structurale)

Journal (Biochemistry)

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Melaku Garsamo³, Yun Zhou³, Xing Liu³

¹Department of Biochemistry,Purdue University, ²Department of Botany and Plant Pathology,Purdue University, ³Center for Plant Biology,Purdue University

Journal (Biochemistry)

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters

Sebastian Flores, Andrew P. Feld, Bryan H. Thurtle-Schmidt

¹Department of Biology,Davidson College

Journal (Biochemistry)

Quantification of Hypopigmentation Activity In Vitro

Yeon-Ji Kim, Min-Jung Kim, Dong-Keon Kweon, Seung-Taik Lim, Sung-Joon Lee

¹Department of Biotechnology, Graduate School of Life Sciences & Biotechnology, College of Life Sciences and Biotechnology,Korea University, ²JinWoo Bio Co. Ltd

Journal (Biochemistry)

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Sujin Kim, Minjeong Kang, Yoosik Kim

¹Department of Chemical and Biomolecular Engineering and KI for Health Science and Technology (KIHST),Korea Advanced Institute of Science and Technology (KAIST)

Journal (Biochemistry)

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Zackary N. Scholl, Qing Li, Eric Josephs, Dimitra Apostolidou, Piotr E. Marszalek

¹Mechanical Engineering and Materials Science,Duke University

Journal (Biochemistry)

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells

Dominik P. Buser, Martin Spiess

¹Biozentrum,University of Basel

Journal (Biochemistry)

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

John E. Chojnowski, Emily A. McMillan, Todd I. Strochlic

¹Department of Biochemistry and Molecular Biology,Drexel University College of Medicine

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