JOVE JOURNAL

生物化学

Showing 505 - 516 in 724 videos.

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

Rongbing Huang*^1,2, Mengting Han*^1,2, Liying Meng³, Xing Chen^2,3,4,5

¹College of Chemistry and Molecular Engineering,Peking University, ²Beijing National Laboratory for Molecular Sciences,Peking University, ³Peking-Tsinghua Center for Life Sciences,Peking University, ⁴Synthetic and Functional Biomolecules Center,Peking University, ⁵Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Peking University

Journal (Biochemistry)

Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans

Sumino Yanase², Kayo Yasuda, Naoaki Ishii

¹Department of Health Science, School of Sports & Health Science,Daito Bunka University, ²Department of Molecular Life Science, School of Medicine,Tokai University, ³Department of Health Management, School of Health Study,Tokai University

Journal (Biochemistry)

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Chayan Bhattacharya*¹, Aninda Sundar Dey*¹, Navid J. Ayon*¹, William G. Gutheil, Mridul Mukherji

¹Division of Pharmaceutical Sciences, School of Pharmacy,University of Missouri-Kansas City

Journal (Biochemistry)

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

Antonio E. Chambers*¹, Adam E. Richardson*¹, David F. Read, Thomas J. Waller, Douglas A. Bernstein, Philip J. Smaldino

¹Department of Biology,Ball State University, ²Department of Genome Sciences,University of Washington, ³Department of Molecular, Cellular, and Developmental Biology,University of Michigan

Journal (Biochemistry)

Rapid Isolation of the Mitoribosome from HEK Cells

Shintaro Aibara, Juni Andréll, Vivek Singh, Alexey Amunts

¹Science for Life Laboratory, Department of Biochemistry and Biophysics,Stockholm University

Journal (Biochemistry)

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Stacy N. Uchendu, Angelika Rafalowski, Erin F. Cohn, Luke W. Davoren, Erika A. Taylor

¹Department of Chemistry,Wesleyan University

Journal (Biochemistry)

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions

Roberto Pech-Kú, J. Armando Muñoz-Sánchez, Miriam Monforte-González, Felipe Vázquez-Flota, Beatriz A. Rodas-Junco, S.M. Teresa Hernández-Sotomayor

¹Unidad de Bioquímica y Biología Molecular de Plantas,Centro de Investigación Científica de Yucatán, ²CONACYT, Facultad de Ingeniería Química, Campus de Ciencias Exactas e Ingeniería,Universidad Autónoma de Yucatán

Journal (Biochemistry)

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

Anthony Asher, Iniyan Ganesan, Laura Klasek, Steven M. Theg

¹Department of Plant Biology,University of California - Davis

Journal (Biochemistry)

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

Marjaana Rantala, Virpi Paakkarinen, Eva-Mari Aro

¹Molecular Plant Biology, Department of Biochemistry,University of Turku

Journal (Biochemistry)

Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts

Ye Guan², Shiyuan Wang, Minjun Jin, Haotian Xu, Qiong Yang⁴

¹Department of Biophysics,University of Michigan, Ann Arbor, ²Department of Chemistry,University of Michigan, Ann Arbor, ³Department of Computer Science,Wayne State University, ⁴Department of Physics,University of Michigan, Ann Arbor

Journal (Biochemistry)

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Narendra Padhan

¹Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory,751 85, Uppsala, Sweden

Journal (Biochemistry)

Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee

Shota Suenami², Ryo Miyazaki, Takeo Kubo

¹Bioproduction Research Institute,National Institute of Advanced Industrial Science and Technology, ²Department of Biological Sciences, Graduate School of Science,The University of Tokyo

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