JOVE JOURNAL

生物化学

Showing 553 - 564 in 724 videos.

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Kang-Yi Su*^1,2, Steven D. Goodman*³, Hung-Ming Lai, Rong-Syuan Yen, Wei-Yao Hu, Wern-Cherng Cheng, Liang-In Lin², Ya-Chien Yang², Woei-Horng Fang²

¹Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine,National Taiwan University, ²Department of Laboratory Medicine,National Taiwan University Hospital, ³Center for Microbial Pathogenesis, Nationwide Children's Hospital and the Department of Pediatrics,The Ohio State University

Journal (Biochemistry)

Defining Hsp33’s Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

Rosi Fassler*¹, Nufar Edinger*¹, Oded Rimon, Dana Reichmann

¹Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, Safra Campus Givat Ram,The Hebrew University of Jerusalem

Journal (Biochemistry)

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Tabitha A. Peterson, Mark A. Stamnes, Robert C. Piper

¹Molecular Physiology and Biophysics,University of Iowa

Journal (Biochemistry)

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C

Cuiping Li, Weiyi Lai, Hailin Wang

¹State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences

Journal (Biochemistry)

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

Hiroyuki Yamamoto, Yoshikazu Sawaguchi, Michio Kimura

¹Department of Microbiology and Molecular Cell Biology,Nihon Pharmaceutical University, ²Department of Clinical Pharmaceutics,Nihon Pharmaceutical University

Journal (Biochemistry)

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis

Huang Chunhui², Xu Dilin, Zhang Ke, Shentu Jieyi, Yan Sicheng, Wu Dapeng, Wang Qinwen, Cui Wei²

¹Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine,Ningbo University, ²Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center,Ningbo University, ³School of Materials Science and Chemical Engineering,Ningbo University

Journal (Biochemistry)

Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs

Joseph A. Ross, Nehal Thakor^2,3

¹Department of Chemistry and Biochemistry, Alberta RNA Research and Training Institute,University of Lethbridge, ²Department of Neuroscience and the Canadian Centre for Behavioral Neuroscience (CCBN),University of Lethbridge, ³Arnie Charbonneau Cancer Institute,University of Calgary

Journal (Biochemistry)

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Kotaro Hama, Yuko Fujiwara, Kazuaki Yokoyama

¹Faculty of Pharmaceutical Sciences,Teikyo University

Journal (Biochemistry)

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Shannon C. Kelly*¹, Amanda M. Eccardt*¹, Jonathan S. Fisher

¹Department of Biology,Saint Louis University

Journal (Biochemistry)

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Heying Cui*¹, Kyle M. Loftus*¹, Crystal R. Noell, Sozanne R. Solmaz

¹Department of Chemistry,State University of New York at Binghamton

Journal (Biochemistry)

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

Joshua T. Maxwell, Chin-Hsien Tsai, Tahmina A. Mohiuddin, Jennifer Q. Kwong

¹Department of Pediatrics,Emory University School of Medicine, ²Emory College of Arts and Sciences,Emory University

Journal (Biochemistry)

Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers

Sarah Hanna-Addams, Zhigao Wang

¹Department of Molecular Biology,UT Southwestern Medical Center

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