JOVE JOURNAL

生物化学

Showing 649 - 660 in 724 videos.

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization

Xavier Morató*^1,2, Marc López-Cano*^1,2, Paula M. Canas, Rodrigo A. Cunha, Francisco Ciruela²

¹Unitat de Farmacologia, Departament Patologia i Terapèutica Experimental, Facultat de Medicina, IDIBELL,Universitat de Barcelona, L'Hospitalet de Llobregat, ²Institut de Neurociències,Universitat de Barcelona, ³Center for Neurosciences of Coimbra, Institute of Biochemistry, Faculty of Medicine,University of Coimbra

Journal (Biochemistry)

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples

Tyler Rhinesmith, Bryan A. Killinger, Akhil Sharma, Anna Moszczynska

¹Physiology,Michigan State University, ²Center for Neurodegenerative Science Van Andel Institute, ³Pharmaceutical Sciences,Wayne State University

Journal (Biochemistry)

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification

Melissa Bredow, Heather E. Tomalty, Virginia K. Walker^2,3

¹Department of Biology,Queen's University, ²Department of Biomedical and Molecular Sciences,Queen's University, ³School of Environmental Sciences,Queen's University

Journal (Biochemistry)

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

Christina D. King, Joseph D. Dudenhoeffer, Liqing Gu, Adam R. Evans, Renã A. S. Robinson

¹Department of Chemistry,University of Pittsburgh, ²SGS North America Inc., ³Large Molecule Analytical Development, Pharmaceutical Development & Manufacturing Science,Janssen Research and Development

Journal (Biochemistry)

Modification and Functionalization of the Guanidine Group by Tailor-made Precursors

Tobias G. Kapp*¹, Maximilian Fottner*¹, Horst Kessler

¹Department of Chemistry,Technische Universität München

Journal (Biochemistry)

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Javier Barallobre-Barreiro, Ferheen Baig, Marika Fava, Xiaoke Yin, Manuel Mayr

¹King's British Heart Foundation Centre,King's College London

Journal (Biochemistry)

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Danlin Yang, Ajit Singh, Helen Wu, Rachel Kroe-Barrett

¹Department of Biotherapeutics Discovery, Immune Modulation and Biotherapeutics Discovery,Boehringer Ingelheim Pharmaceuticals, Inc., ²The Fu Foundation School of Engineering and Applied Science,Columbia University

Journal (Biochemistry)

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Kathrin Rösch, Marcel Kwiatkowski, Hartmut Schlüter, Eva Herker

¹Heinrich Pette Institute,Leibniz Institute for Experimental Virology, ²Core Facility Mass Spectrometric Proteomics,University Medical Center Hamburg-Eppendorf

Journal (Biochemistry)

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Cristina Lento, Shaolong Zhu, Kerene A. Brown, Ruth Knox, Peter Liuni, Derek J. Wilson^2,3

¹Department of Chemistry,York University, ²The Centre for Research in Mass Spectrometry,York University, ³The Centre for Research on Biomolecular Interactions,York University

Journal (Biochemistry)

LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics

Xavier Gallart-Palau*¹, Aida Serra*¹, Siu Kwan Sze

¹Division of Structural Biology and Biochemistry, School of Biological Sciences,Nanyang Technological University

Journal (Biochemistry)

Hydrolysis of a Ni-Schiff-Base Complex Using Conditions Suitable for Retention of Acid-labile Protecting Groups

Cory A. Bontrager, Tanner J. Geibel, George A. Lengyel

¹Department of Chemistry,Slippery Rock University

Journal (Biochemistry)

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Mercedes Pardo, Daniel Bode, Lu Yu, Jyoti S. Choudhary

¹Proteomic Mass Spectrometry,Wellcome Trust Sanger Institute

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