JOVE JOURNAL

癌症研究

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A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Alexander R. Fuchs, Melanie Märklin, Jonas S. Heitmann, Stefan Futterknecht, Michael Haap, Stefan Wirths, Hans-Georg Kopp, Clemens Hinterleitner, Daniela Dörfel, Martin R. Müller

¹Dept. of Hematology, Oncology and Immunology,University of Tübingen, ²Dept. of Endocrinology, Diabetology, Clinical Pathology and Metabolism,University of Tübingen

Journal (Cancer Research)

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation

Andreas Agathangelidis*¹, Lesley Ann Sutton*^2,3, Anastasia Hadzidimitriou, Cristina Tresoldi, Anton W. Langerak, Chrysoula Belessi, Frederic Davi, Richard Rosenquist³, Kostas Stamatopoulos², Paolo Ghia

¹Institute of Applied Biosciences,Centre for Research and Technology Hellas, ²Department of Immunology, Genetics and Pathology, Science for Life Laboratory,Uppsala University, ³Department of Molecular Medicine and Surgery,Karolinska Institutet, ⁴Division of Immunology, Transplantation and Infectious,IRCCS San Raffaele Scientific Institute, ⁵Department of Immunology, Laboratory for Medical Immunology,Erasmus University Medical Center, ⁶Hematology Department,Nikea General Hospital, ⁷Assistance publique - Hôpitaux de Paris (AP-HP), Hopital Pitié-Salpêtrière, Department of Hematology, and UPMC University Paris 06, UMRS 1138, ⁸Division of Experimental Oncology,IRCCS Istituto Scientifico San Raffaele and Università Vita-Salute San Raffaele

Journal (Cancer Research)

Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer’s Patches

Yavuz F. Yazicioglu², Halil I. Aksoylar², Rinku Pal², Nikolaos Patsoukis², Vassiliki A. Boussiotis²

¹Division of Hematology-Oncology, Beth Israel Deaconess Medical Center,Harvard Medical School, ²Department of Medicine, Beth Israel Deaconess Medical Center,Harvard Medical School

Journal (Cancer Research)

Assessment of the Metabolic Profile of Primary Leukemia Cells

Katerina Hlozková, Julia Starková

¹CLIP - Childhood Leukemia Investigation Prague, Second Faculty of Medicine, Laboratory of Molecular Genetics,Charles University, Prague

Journal (Cancer Research)

Morphology-Based Distinction Between Healthy and Pathological Cells Utilizing Fourier Transforms and Self-Organizing Maps

Fabian L. Kriegel², Ralf Köhler, Jannike Bayat-Sarmadi, Simon Bayerl, Anja E. Hauser³, Raluca Niesner, Andreas Luch*¹, Zoltan Cseresnyes*⁴

¹Department of Chemical and Product Safety,German Federal Institute for Risk Assessment (BfR), ²Deutsches Rheuma-Forschungszentrum (DRFZ) Berlin, a Leibniz Institute, ³Charité Universitätsmedizin Berlin, ⁴Applied Systems Biology,Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute

Journal (Cancer Research)

Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array (HMCA)-based Plates

Orit Ravid-Hermesh, Naomi Zurgil, Yana Shafran, Elena Afrimzon, Maria Sobolev, Yaron Hakuk, Zehavit Bar-On Eizig, Mordechai Deutsch

¹Physics Department,Bar-Ilan University

Journal (Cancer Research)

A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells

Gray Kueberuwa, Weiming Zheng, Milena Kalaitsidou, David E. Gilham², Robert E. Hawkins

¹Manchester Cancer Research Centre Building, Department Cancer Sciences,University of Manchester, ²Celyad

Journal (Cancer Research)

Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances

Xin Feng, Shaofei Wang, Quewang Liu, Han Li, Jiamei Liu, Cheng Xu, Weifeng Yang, Yayun Shu, Weiwei Zheng, Bingxin Yu, Mingran Qi, Wenyang Zhou, Fengfeng Zhou

¹College of Computer Science and Technology, and Key Laboratory of Symbolic Computation and Knowledge Engineering of Ministry of Education,Jilin University, ²College of Software,Jilin University, ³Ultrasonography Department,China-Japan Union Hospital of Jilin University, ⁴Department of Pathogenobiology, College of Basic Medical Science,Jilin University

Journal (Cancer Research)

Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry

Xiaoyu An*^1,2, Xuesong Ouyang*¹, Hui Zhang, Tingting Li, Yu-yang Huang, Zhiyuan Li, Demin Zhou, Qi-Xang Li²

¹Crown Bioscience Inc., ²State Key Laboratory of Natural and Biomimetic Drugs,Peking University

Journal (Cancer Research)

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

Sigrid S. Skånland²

¹Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership,University of Oslo, ²K. G. Jebsen Centre for B cell malignancies and K. G. Jebsen Centre for Cancer Immunotherapy,University of Oslo

Journal (Cancer Research)

Using 22C3 Anti-PD-L1 Antibody Concentrate on Biopsy and Cytology Samples from Non-small Cell Lung Cancer Patients

Marius Ilié^2,3, Mélanie Ngo-Mai, Elodie Long-Mira², Sandra Lassalle², Catherine Butori, Coraline Bence, Marame Hamila, Véronique Hofman^2,3, Paul Hofman^2,3

¹Laboratory of Clinical and Experimental Pathology, Pasteur Hospital, Hospital University Federation OncoAge,Université Côte d’Azur, ²Institute for Research on Cancer and Aging in Nice (Inserm U1081 and CNRS 7284),Université Côte d’Azur, ³Hospital-Integrated Biobank (BB-0033-00025), Pasteur Hospital,Université Côte d’Azur

Journal (Cancer Research)

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays

Katrin Tiemann, Carolina Garri, Jeffrey Wang, Lauren Clarke, Kian Kani

¹Lawrence J. Ellison Institute for Transformative Medicine, Center for Applied Molecular Medicine,University of Southern California

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