dATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA - JoVE

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DATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA (Video) | JoVE

Published: April 21, 2023

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Llorenç Rovirosa*¹, Laureano Tomás-Daza*^1,2, Blanca Urmeneta, Alfonso Valencia³, Biola M. Javierre⁴

¹Josep Carreras Leukaemia Research Institute, ²Barcelona Supercomputing Center, ³Catalan Institution for Research and Advanced Studies (ICREA), ⁴Institute for Health Science Research Germans Trias i Pujol

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Keywords: DATP-Tailing Adapter Ligation PCR Amplification Scarce Cell DNA Biotin Klenow Exo-minus Ligation Buffer T4 DNA Ligase Paramagnetic Bead Purification Double-Sided Selection TLE Buffer 70% Ethanol

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dATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA. J. Vis. Exp. (Pending Publication), e200139, doi:10.3791/200139 (2023).

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