Pancreatic Intravital Imaging Window Installation in Mouse Model: An Integrated Pancreatic Imaging Window for Time-lapse In Vivo Imaging of Murine Pancreas Microstructure

Published: April 30, 2023

Abstract

Source: Park, I. et. al. Stabilized Longitudinal In Vivo Cellular-Level Visualization of the Pancreas in a Murine Model with a Pancreatic Intravital Imaging Window. J. Vis. Exp. (2021)

This video demonstrates the procedure to implant a pancreatic imaging window to separate the pancreas from the intestines for longitudinal time-lapse high-resolution imaging of the pancreas microstructure.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Surgery Prepare a sterile surgical platform and sterilize the surfaces with 70% ethanol.| NOTE: For longitudinal imaging sessions, aseptic technique is essential. Anesthetize mice with a mixture of tiletamine/zolazepam (30 mg/kg) and xylazine (10 mg/kg). NOTE: Use of tiletamine/z…

Representative Results

Figure 1. Design and photograph of the pancreatic intravital imaging window. (A) A 3D and cross-sectional view of the pancreatic intravital imaging window. (B) Anterior and posterior photograph of the pancreatic imaging window.

Disclosures

The authors have nothing to disclose.

Materials

Cover glasses circular Marienfeld 111520 Cover glass for pancreatic imaging window
IRIS Scissor JEUNGDO BIO & PLANT CO, LTD S-1107-10 This product can be replaced with the product from other company
Loctite 401 Henkel 401 N-butyl cyanoacrylate glue
Micro Needle holder JEUNGDO BIO & PLANT CO, LTD H-1126-10 This product can be replaced with the product from other company
Micro rectractor JEUNGDO BIO & PLANT CO, LTD 17004-03 This product can be replaced with the product from other company
Microforceps JEUNGDO BIO & PLANT CO, LTD F-1034 This product can be replaced with the product from other company
Nylon 4-0 AILEE NB434 Non-Absorbable Suture
Omnican N 100 30G B BRAUN FT9172220S For Vascular Catheter, Use only Needle part
Pancreatic imaging window Geumto Engineering Custom order Pancreatic imaging window – custom order
Physiosuite Kent Scientific PS-02 Homeothermic temperature controller
Ring Forceps JEUNGDO BIO & PLANT CO, LTD F-1090-3 This product can be replaced with the product from other company
Rompun Bayer Rompun Anesthetic agent
Zoletil Virbac Zoletil 100 Anesthetic agent
BALB/C Nude  OrientBio  BALB/C Nude BALB/C Nude

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Cite This Article
Pancreatic Intravital Imaging Window Installation in Mouse Model: An Integrated Pancreatic Imaging Window for Time-lapse In Vivo Imaging of Murine Pancreas Microstructure. J. Vis. Exp. (Pending Publication), e20796, doi: (2023).

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