Masson Goldner Trichrome Staining: A Technique to Visualize Collagen in Heart Sections for Cardiovascular Fibrosis Detection
Published: April 30, 2023
Abstract
Source: Valentin, J., et al. Histological Quantification of Chronic Myocardial Infarct in Rats. J. Vis. Exp. (2016).
This video demonstrates the differential staining of collagen fibers, nuclei, and cytoplasm in heart tissue to visualize fibrotic growth associated with cardiac pathologies.
Protocol
1. Masson-Goldner Trichrome Staining Make tissue sections from paraffin-embedded heart blocks with a manual microtome (thickness: 5 µm). Stain one slide from each heart part for each rat (3 – 4 transversal sections per slide) with Masson-Goldner trichrome staining. Melt the slides at 60 °C in an oven. Under a fume hood, deparaffinize them in xylol twice for 10 min each. Rehydrate them in 100% ethanol, 95% ethanol, 70% ethanol, and distilled water for 3 min each. Fix …
Disclosures
The authors have nothing to disclose.
Materials
| Mayer hematoxylin | MHS32-1L | Sigma Aldrich | |
| Acid Fuchsin CI 42685 | F8129-50G | Sigma Aldrich | |
| Ponceau Xylidin CI 16150 | P2395-25G | Sigma Aldrich | |
| Orange G CI 16230 | O3756-100G | Sigma Aldrich | |
| Light green CI 42095 | L5382-25G | Sigma Aldrich | |
| Xylol | 10315083 | HoneyWell | |
| Ethanol absolute | 10303990 | HoneyWell | |
| Sterelogical microscope | SM2800 | Nikon | |
| Formaldehyde | 99340 | Reactolab | |
| Embedding cassette | K113.1 | Carl Roth | |
| Bersoft Image measurement Software | Bersoft.com | Licensed software |
Tags
Cite This Article
Masson Goldner Trichrome Staining: A Technique to Visualize Collagen in Heart Sections for Cardiovascular Fibrosis Detection. J. Vis. Exp. (Pending Publication), e21053, doi: (2023).