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Optimized Magnetic High-Yield Production of Endosome-Derived Nanovesicles

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배양된 세포에서 자성 나노입자를 함유한 엔도솜의 분리 및 준비

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生物学

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JoVE 杂志生物学

Isolation and Preparation of Endosomes Containing Magnetic Nanoparticles from Cultured Cells

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배양된 세포에서 자성 나노입자를 함유한 엔도솜의 분리 및 준비

Optimized Magnetic High-Yield Production of Endosome-Derived Nanovesicles

DOI:

10.3791/200760-v

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03:39 min

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January 26, 2024

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Dujuan Wang², Shili Yao³, Peng Guo^2,3,4

¹Academy of Medical Engineering and Translational Medicine,Tianjin University, ²Hangzhou Institute of Medicine (HIM),Chinese Academy of Sciences, ³School of Materials Science and Engineering,Tianjin University, ⁴Zhejiang Cancer Hospital

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Endosomes Magnetic Nanoparticles Cell Culture DMEM Medium Trypsin Hypotonic Solution Homogenization Magnetic Separation High-speed Homogenization Exosome Mimics NTA TEM Western Blotting

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