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Optimized Magnetic High-Yield Production of Endosome-Derived Nanovesicles
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Isolamento e Preparação de Endossomos Contendo Nanopartículas Magnéticas a partir de Células Cultivadas
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生物学
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JoVE 杂志 生物学
Isolation and Preparation of Endosomes Containing Magnetic Nanoparticles from Cultured Cells

Isolamento e Preparação de Endossomos Contendo Nanopartículas Magnéticas a partir de Células Cultivadas

Optimized Magnetic High-Yield Production of Endosome-Derived Nanovesicles

DOI:
10.3791/200760-v

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03:39 min

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January 26, 2024

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, ,
1Academy of Medical Engineering and Translational Medicine,Tianjin University, 2Hangzhou Institute of Medicine (HIM),Chinese Academy of Sciences, 3School of Materials Science and Engineering,Tianjin University, 4Zhejiang Cancer Hospital

Tags

Keywords: EndosomesMagnetic NanoparticlesCell CultureDMEM MediumTrypsinHypotonic SolutionHomogenizationMagnetic SeparationHigh-speed HomogenizationExosome MimicsNTATEMWestern Blotting

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