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Immunology and Infection

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JoVE Journal Immunology and Infection
Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples
 
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Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples

Article DOI: 10.3791/2687-v • 05:24 min • May 10th, 2011
May 10th, 2011 •
1, 1
1Department of Basic Sciences, Mississippi State University

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Here we describe a procedure allowing the labeling of Edwardsiella ictaluri in situ in histological sections from channel catfish Ictalurus punctatus using indirect immunohistochemistry with monoclonal antibodies Ed9 as a primary, and fluorescent FitC labeled antibodies as a secondary. This allowed for the detection of the bacterium using fluorescent microscopy.

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Fluorescent Immuno-Chemistry Detection Edwardsiella Ictaluri Channel Catfish Ictalurus Punctatus Method Development In Situ Visualization Bacteria Histological Sections Bacterial Localization Plate Counts Radiometric Labeled Bioluminescent Bacteria Gross Organ Level Infection Cycle Virulence Factors Interaction With Host Salmonella Typhi Taxonomic Family Technique Description Indirect Immuno-histochemistry Monoclonal Ed9 Antibody Blocking Serum Paraffin Embedded Histological Sections Primary Antibody Secondary Antibodies FitC Labeled Secondary Antibodies
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