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Immunology and Infection

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JoVE Journal Immunology and Infection
Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
 

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

Article DOI: 10.3791/3076-v • 12:50 min • August 9th, 2011
August 9th, 2011 •
1, 2, 2, 1
1St Vincent’s Institute, Department of Medicine, The University of Melbourne, 2Department of Microbiology and Immunology, The University of Melbourne

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Automatic Translation

Listeria monocytogenes is a model organism for studying immune responses and genetic susceptibility to intracellular bacteria in mice. This method enables one to measure bacterial load and generate single-cell suspensions of the liver and spleen from mice for FACS analysis to determine changes in immune cells due to Listeria infection.

Tags

Bacterial Load Immune Responses Mice Listeria Monocytogenes Gram-positive Intracellular Pathogen Systemic Infection Spleen Liver CD8α+ Dendritic Cells Kupffer Cells Phagocytosed Bacterial Proteins Phagosome Cytosol Innate Immune Cells Monocytes Neutrophils NK Cells Dendritic Cells Bactericidal Mechanisms CD8+ T Cells Clearance Of Listeria Host Immune Responses Inbred Mouse Strains Genetic Studies
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