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Journal / Immunology and Infection / Live Cell Imaging of Bacillus subtilis and…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy
 
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Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy

Article DOI: 10.3791/3145-v • 07:32 min • July 28th, 2011
July 28th, 2011 •
1, 1, 1, 1
1Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen

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This protocol provides a step-by-step procedure to monitor single cell behavior of different bacteria in time using automated fluorescence time-lapse microscopy. Furthermore, we provide guidelines how to analyze the microscopy images.

Tags

Live Cell Imaging Bacillus Subtilis Streptococcus Pneumoniae Automated Time-lapse Microscopy Single Cell Studies Microscopy Method Fluorescence Microscopy Growth And Division Phylogenetic Lineage Trees Cell History And Ancestry Gene Expression Dynamics Protein Localization
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