JoVE Journal
Immunology and Infection
Immunology and Infection
需要订阅 JoVE 才能查看此 内容. 登录或开始免费试用。
章节
总结概括
Microbial biofilms are generally constituted by distinct subpopulations of specialized cells. Single-cell analysis of these subpopulations requires the use of fluorescent reporters. Here we describe a protocol to visualize and monitor several subpopulationswithin B. subtilis biofilms using fluorescence microscopy and flow cytometry.