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A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation
JoVE 杂志
生物学
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JoVE 杂志 生物学
A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation
DOI:

10:43 min

May 18, 2013

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Chapters

  • 00:01Title
  • 01:42Protocol for DNA Extraction from P100 Tubes
  • 03:13Lyse Red Blood Cells
  • 03:56Precipitate Protein
  • 04:13Prepare Isopropanol Tubes
  • 05:40Resuspend DNA in DNA Hydration Buffer
  • 06:33Representative Results
  • 08:20Comparison to Alternative Methods
  • 09:45Conclusion

Summary

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We are describing a new method of isolating genomic DNA from whole blood collected for plasma/serology. After plasma collection, the compacted blood is usually discarded. Our novel method represents a significant improvement over existing methods and makes DNA and plasma available from a single collection, without requesting additional blood.

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