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免疫组化分析大鼠中枢神经系统和周围淋巴结组织切片
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神经科学
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JoVE 杂志 神经科学
Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
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免疫组化分析大鼠中枢神经系统和周围淋巴结组织切片

DOI:
10.3791/50425-v

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09:11 min

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November 14, 2016

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, , , , ,
1Department of Clinical Neuroscience, Neuroimmunology Unit, Center for Molecular Medicine,Karolinska Institutet, 2Department of Neuroimmunology, Center for Brain Research,Medical University of Vienna, 3Department of Medical Biochemistry and Biophysics, Vascular Biology Unit,Karolinska Institutet

Chapters

  • 00:05Title
  • 01:46Tissue Preparation: Fixation, Dehydration and Embedding
  • 02:50Tissue Sectioning, Mounting and Deparaffinization
  • 04:32Antigen Retrieval, Blocking, and Incubation with the Primary Antibodies
  • 06:38Visualization of the Bound Biotinylated Secondary Antibody
  • 07:48Results: Double Immunostaining in the Rat Lymph Nodes and the Central Nervous System
  • 08:42Conclusion

Summary

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We here present an optimized, detailed protocol for double immunostaining in formalin-fixed, paraffin-embedded rat central nervous system (CNS) and peripheral lymph node (LN) tissue sections.

Tags

ImmunohistochemistryRat Central Nervous SystemPeripheral Lymph NodeProtein LocalizationParaffin-embeddingAntigen RetrievalAntibodiesSignal DetectionLight Microscopy

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