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Immunohistochemical ניתוח של מערכת העצבים המרכזית עכברוש לימפה הפריפריה צומת סעיפים רקמות
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
DOI:

09:11 min

November 14, 2016

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Chapters

  • 00:05Title
  • 01:46Tissue Preparation: Fixation, Dehydration and Embedding
  • 02:50Tissue Sectioning, Mounting and Deparaffinization
  • 04:32Antigen Retrieval, Blocking, and Incubation with the Primary Antibodies
  • 06:38Visualization of the Bound Biotinylated Secondary Antibody
  • 07:48Results: Double Immunostaining in the Rat Lymph Nodes and the Central Nervous System
  • 08:42Conclusion

Summary

自动翻译

We here present an optimized, detailed protocol for double immunostaining in formalin-fixed, paraffin-embedded rat central nervous system (CNS) and peripheral lymph node (LN) tissue sections.

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