Journal
/
化学
/
A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence
JoVE 杂志
化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 化学
A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence

A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence

DOI:
10.3791/51441-v

•

10:56 min

•

May 30, 2014

•

, , ,
1Department of Chemical and Materials Engineering,University of Alberta, 2Department of Chemical and Petroleum Engineering,University of Calgary

Chapters

  • 00:05Title
  • 01:52Isolation of Dry Algal Biomass to be Used as Standards for Fluorescence Readings
  • 02:52Gravimetric Quantification of Neutral Lipids by Hexane Extraction
  • 04:48Fluorometric Quantification of Neutral Lipids Using Nile Red
  • 06:42Fluorescence Microscopy Technique
  • 08:04Results: Images and Calibration Curves of A. protothecoides Stained with Nile Red
  • 10:18Conclusion

Summary

自动翻译

自动翻译

A simple protocol to determine the neutral lipid content of algal cells using a Nile Red staining procedure is described. This time-saving technique offers an alternative to traditional gravimetric-based lipid quantification protocols. It has been designed for the specific application of monitoring bioprocess performance.

Tags

Algal CellsNeutral LipidsFluorescenceNile RedAuxenochlorella ProtothecoidesLipid ContentBioprocess MonitoringRapid ProtocolGravimetric MethodsCell Membrane Permeabilization

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

A Protocol for the Production of Gliadin-cyanoacrylate Nanoparticles for Hydrophilic Coating

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

A Simple and Efficient Protocol for the Catalytic Insertion Polymerization of Functional Norbornenes

A Protocol for Safe Lithiation Reactions Using Organolithium Reagents

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group

Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS

Quantifying X-Ray Fluorescence Data Using MAPS

Fabrication and Testing of Catalytic Aerogels Prepared Via Rapid Supercritical Extraction

Read Article