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로봇 미세 접촉 인쇄 (R-μCP)를 사용하여 복합 문화 기판 및 순차 친 핵성 치환 제조
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution
DOI:

08:23 min

October 31, 2014

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Chapters

  • 00:05Title
  • 01:49Surface-initiated Atom Transfer Radical Polymerization (SI-ATRP) of PEGMEMA
  • 03:15Functionalization of Micropatterned PEGMEMA Chains
  • 05:11“Click” Biotinylation of PEGMEMA Chains Including Staining
  • 07:10Results: System Accuracy
  • 07:57Conclusion

Summary

自动翻译

Cell culture substrates functionalized with microscale patterns of biological ligands have immense utility in the field of tissue engineering. Here, we demonstrate the versatile and automated manufacture of tissue culture substrates with multiple, micropatterned poly(ethylene glycol) brushes presenting orthogonal chemistries that enable spatially precise and site-specific immobilization of biological ligands.

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