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Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
 

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Article DOI: 10.3791/52691-v • 07:36 min • May 1st, 2015
May 1st, 2015 •
1,2, 1,3, 1, 1,2,3, 1,2, 1,2,3
1Murdoch Childrens Research Institute, The Royal Children’s Hospital, 2Department of Paediatrics, Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne, 3Department of Anatomy and Developmental Biology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton

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The goal of this protocol is to isolate lymphatic endothelial cells lining human lymphatic malformation cyst-like vessels and foreskins using fluorescence-activated cell sorting (FACS). Subsequent cell culturing and expansion of these cells permits a new level of experimental sophistication for genetic, proteomic, functional and cell differentiation studies.

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Isolation Human Lymphatic Endothelial Cells Multi-parameter Fluorescence-activated Cell Sorting Lymphatic System Disorders Primary Lymphedema Lymphatic Malformations Lymphatic Tumors Genetic Studies Molecular Studies Cellular Studies New Therapies Clinical Management Protocol Single Cell Suspension Tissue Mincing Enzymatic Treatment Dispase II Collagenase II Cluster Of Differentiation Markers CD34 CD31 Vascular Endothelial Growth Factor-3 (VEGFR-3) PODOPLANIN Fluorescently Activated Cell Sorter (FACS) LM LEC Population Endothelial Cells
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