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在锝-99m在马腱末端标记的骨髓间充质干细胞的体内成像和跟踪
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医学
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JoVE 杂志 医学
In Vivo Imaging and Tracking of Technetium-99m Labeled Bone Marrow Mesenchymal Stem Cells in Equine Tendinopathy
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在锝-99m在马腱末端标记的骨髓间充质干细胞的体内成像和跟踪

DOI:
10.3791/52748-v

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07:52 min

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December 09, 2015

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, , , , ,
1Department of Clinical Science and Services,The Royal Veterinary College, 2Hospital de Referencia La Equina, Manilva, 3Department of Nuclear Medicine,Barts & The London NHS Trust

Chapters

  • 00:05Title
  • 01:01Equine Forelimb Tendon Ultrasonography and Mesenchymal Stem Cell Tc-99m Labeling
  • 03:31Tc-99m-HMPAO-labeled Cell Intralesional Implantation
  • 04:29Gamma Scintigraphy Imaging
  • 05:24Results: Representative Recorded Radioactivity after Intralesional Injection
  • 06:54Conclusion

Summary

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This protocol describes the radiolabeling of equine mesenchymal stem cells and their implantation into tendon injuries in the horse in order to determine cell survival and tissue distribution using gamma scintigraphy.

Tags

In Vivo ImagingTechnetium-99mBone Marrow Mesenchymal Stem CellsEquine TendinopathyCell TrackingGamma ScintigraphyHMPAO

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