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Chapters
- 00:05Title
- 00:32Mononuclear Cell (MNC) Isolation from Human Palatine Tonsils
- 02:45Tonsillar T Lymphocyte Positive Selection
- 04:24Isolated Tonsillar B and T Lymphocyte Flow Cytometric and PCR Analysis
- 05:39Results: Representative Tonsillar B and T Lymphocyte Isolation
- 06:34Conclusion
Palatine tonsils are a rich source of B and T lymphocytes. Here we provide an easy, efficient and rapid protocol to isolate B and T lymphocytes from human palatine tonsils. The method described has been specifically adapted for studies of the viral etiology of tonsil inflammation known as tonsillitis.
