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Developmental Biology

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JoVE Journal Developmental Biology
Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
 

Spatial and Temporal Analysis of Active ERK in the C. elegans Germline

Article DOI: 10.3791/54901-v • 08:40 min • November 29th, 2016
November 29th, 2016 •
1, 1,2
1Program in Developmental Biology, Baylor College of Medicine, 2Department of Genetics, UT MD Anderson Cancer Center

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We present an immunofluorescence imaging-based method for spatial and temporal localization of active ERK in the dissected C. elegans gonad. The protocol described here can be adapted for visualization of any signaling or structural protein in the C. elegans gonad, provided a suitable antibody reagent is available.

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Spatial Analysis Temporal Analysis Active ERK C. Elegans Germline ERK Signaling Germcell Development Drug Treatments Gene Perturbations RAS-ERK Pathway Quantification In Vivo Worm Collection M9 Buffer Centrifuge Wash Bacteria Removal Levamisole Addition Dissection Procedure
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