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JoVE Journal
Genetics

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Chromatin-immunpræcipitation (ChIP) i mus-T-cellelinier
 
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Chromatin-immunpræcipitation (ChIP) i mus-T-cellelinier

Article DOI: 10.3791/55907-v 11:39 min June 17th, 2017
June 17th, 2017

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Dette værk beskriver en protokol for chromatinimmunpræcipitation (ChIP) ved anvendelse af en moden mus-T-cellelinie. Denne protokol er egnet til at undersøge fordelingen af ​​specifikke histonmærker på specifikke promotorsteder eller genom-bred.

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Genetik udgave 124 ChIP H3K4me1 H3K4me3 H3K27ac H3 histonmærker sonikering shearing chromatin regulering transkription.
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