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Journal / Genetics / Forbigående udtryk af fremmede gener i insekt…
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JoVE Journal Genetics
Forbigående udtryk af fremmede gener i insekt celler (sf9) for Protein funktionel analyse
 
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Forbigående udtryk af fremmede gener i insekt celler (sf9) for Protein funktionel analyse

Article DOI: 10.3791/56693-v • 11:12 min • February 22nd, 2018
February 22nd, 2018 •
1, 2, 2, 3, 1
1Department of Biotechnology and Animal Science, National Ilan University, 2Department of Agricultural Biology, College of Agriculture Life Sciences, Chonbuk National University, 3Department of Entomology, National Taiwan University

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Denne protokol beskriver en heat shock-induceret protein udtryk system (pDHsp/V5-hans/sf9 celle system), som kan bruges til enten at udtrykke fremmede proteiner eller evaluere det anti-apoptotiske aktivitet af potentielle udenlandske proteiner og deres afkortet amino syrer i insekt celler.

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Genetik sag 132 forbigående udtryk protein funktionel analyse sf9 cell line Lymantria xylina flere nucleopolyhedrovirus Ly-inhinbitor af apoptose-3 Drosophila heat shock 70 promotor pDHsp/V5-hans Drosophilia Reaper actinomycin-D
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