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A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model
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JoVE 杂志 癌症研究
A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model

DOI:
10.3791/57182-v

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08:14 min

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February 28, 2018

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, , , , , , , ,
1Division of Gastroenterology, Hepatology, and Nutrition, Department of Internal Medicine,University of Utah, 2Huntsman Cancer Institute,University of Utah

Chapters

  • 00:04Title
  • 00:50Environmentally Enriched Cage Set Up
  • 02:36Stool Collection at 16 Weeks of Age
  • 03:53Processing gDNA from the Stool
  • 06:00Results: The Effect of Enhanced Environments on Microbiome Biodiversity
  • 07:28Conclusion

Summary

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Environmental Enrichment (EE) is an animal housing environment that is used to reveal mechanisms that underlie the connections between lifestyle, stress, and disease. This protocol describes a procedure that uses a mouse model of colon tumorigenesis and EE to specifically define alterations in microbiota biodiversity that may impact animal mortality.

Tags

Environmental EnrichmentColon MicrobiomeColon Tumor ModelMicrobiome NormalizationExperimental ReproducibilityAnimal Stress ReductionBreedingPup RearingStool Collection

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