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Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
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JoVE 杂志 发育生物学
Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

DOI:
10.3791/57194-v

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12:40 min

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May 19, 2018

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, ,
1Department of Regenerative Medicine and Cell Biology,Medical University of South Carolina

Chapters

  • 00:04Title
  • 01:05Culture of Human Induced Pluripotent Stem Cells
  • 03:39Differentiation of Human iPSCs to Hepatocyte-like Cells and Small Molecule Screening
  • 09:34Results: Differentiation of iPSCs and Identification of Small Molecules for Drug Discovery
  • 11:12Conclusion

Summary

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The protocol presented here describes a platform for identifying small molecules for the treatment of liver disease. A step-by-step description is presented detailing how to differentiate iPSCs into cells with hepatocyte characteristics in 96-well plates, and to use the cells to screen for small molecules with potential therapeutic activity.

Tags

Human Induced Pluripotent Stem CellsHepatocytesLiver DiseaseHigh-throughput ScreeningDrug DiscoveryCell DifferentiationEcadDPBSY-27632EDTAM Medium

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