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Immunology and Infection

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JoVE Journal Immunology and Infection
Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model
 

Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model

Article DOI: 10.3791/57658-v • 07:26 min • July 1st, 2018
July 1st, 2018 •
1,2,3, 1,2,3, 1,2,3,4, 1,2,3, 1,2,3, 1,2,3,5, 1,2,3
1College of Veterinary Medicine, Nanjing Agricultural University, 2Key Lab of Animal Bacteriology, Ministry of Agriculture, 3OIE Reference Lab for Swine Streptococcosis, 4School of Medicine, Tsinghua University, 5Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

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Here, we describe the intracranial subarachnoidal route of infection in mice to study roles of biofilms in Streptococcus suis meningitis. This infection model is also suitable for studying the pathogenesis of other bacterial meningitis and the efficacy of new drugs against bacterial meningitis.

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Intracranial Subarachnoidal Route Of Infection Streptococcus Suis Biofilms Meningitis Mouse Infection Model Bacterial Components Immune Responses Central Nervous System Brain Entrance Procedure Shouming Zhang Graduate Student Strain P1/7 Planktonic Cells Mid-lock Phase Culture Centrifuge PBS Glycerol THB Aliquot Strain P1/7 Biofilm State Cells Overnight Culture THB Round Culture Plates Incubate 37 Degrees Celsius Carbon Dioxide Aspiration
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