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Generating a Murine Orthotopic Metastatic Breast Cancer Model and Performing Murine Radical Mastectomy
 

Generating a Murine Orthotopic Metastatic Breast Cancer Model and Performing Murine Radical Mastectomy

Article DOI: 10.3791/57849-v • 05:05 min • November 29th, 2018
November 29th, 2018 •
1, 1, 2,3,4,5, 1,6,7,8,9,10
1Breast Surgery, Department of Surgical Oncology, Roswell Park Comprehensive Cancer Center, 2Holy Cross Hospital Michael and Dianne Bienes Comprehensive Cancer Center, 3Department of Surgery, Massachusetts General Hospital, 4Department of Surgery, University of Miami Miller School of Medicine, 5Department of Surgery, Nova Southeastern University School of Medicine, 6Department of Surgery, University at Buffalo Jacobs School of Medicine and Biomedical Sciences, 7Department of Breast Surgery and Oncology, Tokyo Medical University, 8Department of Surgery, Yokohama City University, 9Department of Surgery, Niigata University Graduate School of Medical and Dental Sciences, 10Department of Surgery, Fukushima Medical University

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总结概括
Automatic Translation

We introduce a murine orthotopic breast cancer model and radical mastectomy model with bioluminescence technology to quantify the tumor burden to mimic human breast cancer progression.

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Murine Orthotopic Metastatic Breast Cancer Model Murine Radical Mastectomy Breast Cancer Progression Mouse Model Tumorigenesis Rate Local Recurrence Rate Anesthetized Female Mouse Surgical Board Sterile Cotton Swabs Chlorhexidine Iodine 75% Ethanol Skin Incision Anterior Chest Wall Microdissection Scissors Mammary Fat Pad Cancer Cell Suspension Insulin Syringe Gelatinous Cancer Cell Protein Mixture Nonabsorbable Sutures
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