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Biochemistry

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JoVE Journal Biochemistry
Kontrast-matche vaskemiddel i små-Angle Neutron Scattering eksperimenter for membran Protein strukturel analyse og Ab Initio modellering
 
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Kontrast-matche vaskemiddel i små-Angle Neutron Scattering eksperimenter for membran Protein strukturel analyse og Ab Initio modellering

Article DOI: 10.3791/57901-v • 10:27 min • October 21st, 2018
October 21st, 2018 •
1, 2, 1, 1, 2, 1
1Neutron Scattering Division, Oak Ridge National Laboratory, 2School of Chemistry and Biochemistry, Georgia Institute of Technology

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Denne protokol demonstrerer hvordan man kan opnå en lav opløsning ab initio model og strukturelle detaljerne i et vaskemiddel-solubilized membran protein i løsning ved hjælp af små-angle neutron scattering med kontrast-matche vaskemiddel.

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Biokemi spørgsmål 140 lille vinkel spredning neutron scattering Biofysik membran protein kontrast variation overfladeaktivt stof micelle intramembrane aspartyl protease presenilin signal peptid peptidase
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