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Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons
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神经科学
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JoVE 杂志 神经科学
Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons

Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons

DOI:
10.3791/57988-v

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07:43 min

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January 07, 2019

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, ,
1Institut NeuroMyoGène, CNRS UMR5310, INSERM U1217,Université Lyon, 2Unité Fonctionnelle de Neurogénétique Moléculaire,Hospices Civils de Lyon, 3Centre de Biotechnologie Cellulaire,Hospices Civils de Lyon

Chapters

  • 00:04Title
  • 00:36Dissection
  • 01:49Spinal Cord Cell Suspension
  • 02:58Motoneuron Enrichment by Gradient Density
  • 04:42Motoneuron Culture and Magnetofection
  • 06:00Results: Confocal Images of Magnetofected Motoneurons
  • 06:59Conclusion

Summary

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The goal of this technique is to prepare a highly enriched culture of primary motoneurons (MNs) from murine spinal cord. To evaluate the consequences of mutations causing MN diseases, we describe here the isolation of these isolated MNs and their transfection by magnetofection.

Tags

Charcot-Marie-Tooth DiseaseIn Vitro ModelingPrimary Motor NeuronsTransfectionNeurobiologyMotor Neuron PolarityAxon GuidanceAxon TraffickingNeurodegenerative MechanismsEnriched Motor Neuron CultureKnockdown ProteinSpinal Cord DissectionEnzymatic DigestionCell SuspensionDensity Gradient CentrifugationMotoneuron Enrichment

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