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JoVE Journal Genetics
Enkelt dråbe Digital Polymerase Chain Reaction for omfattende og samtidige påvisning af mutationer i Hotspot regioner
 
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Enkelt dråbe Digital Polymerase Chain Reaction for omfattende og samtidige påvisning af mutationer i Hotspot regioner

Article DOI: 10.3791/58051-v • 08:23 min • September 25th, 2018
September 25th, 2018 •
1,2, 1, 1, 1,3,4, 1,3,5, 1,6, 1
1Circulating Tumor Biomarkers Laboratory, SiRIC, Translational Research Department, Institut Curie, PSL Research University, 2CNRS UMR144, Institut Curie, PSL Research University, 3Department of Medical Oncology, Institut Curie, PSL Research University, 4University Versailles Saint-Quentin-en-Yvelines, 5University Paris Descartes, 6Inserm U830, Institut Curie, PSL Research University

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Vi præsenterer her, en protokol for at nøjagtigt kvantificere flere genetiske ændringer af et målområde i en enkelt reaktion ved hjælp af drop-off ddPCR og et unikt par hydrolyse sonder.

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Genetik sag 139 Drop-off ddPCR Hotspot mutationer kræft cirkulerende tumor DNA diagnostik
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