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Biochemistry

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JoVE Journal Biochemistry
Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
 

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator

Article DOI: 10.3791/58224-v • 07:11 min • November 1st, 2018
November 1st, 2018 •
*1,3, *1,3, 1,3, 1,3, 2,3, 1,3, 1,3
1Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation, University of Münster, 2Institute of Biochemistry, University of Münster, 3Cluster of Excellence 'Cells in Motion', University of Münster
* These authors contributed equally

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Here, we present an experimental protocol that can be employed to determine the binding affinities and mode of interaction of label-free phospholipid-binding protein annexin A2 with immobilized solid-supported bilayers (SLB) by simultaneously measuring the mass uptake and the viscoelastic properties of the protein annexin A2.

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Dissipative Microgravimetry Study Binding Dynamics Phospholipid Binding Protein Annexin A2 Solid-supported Lipid Bilayers Quartz Resonator Proteins Lipid Membranes Calcium Dependent Binding Annexins Negatively Charged Phospholipids Label Free Sensitive Quantitative Real Time Micromolecule Assemblies Cells Lipid Solutions Molar Ratio Glass Tubes Organic Solvents Vacuum Lyophilization System Citrate Buffer Temperature Phase Transition Temperature Extruder Polycarbonate Membrane
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